Animal Nutrition
Laboratory Manager: Ms. Engineer Marhamat Ghorbani Zadeh Aqjeh Contact Number 04531505163
All the devices below are under the projects of the professors of the steering group. The equipment of this laboratory includes Soxhlet apparatus, Kjeldahl method, crude fiber measurement, oven, electric furnace, spectrophotometer, types of centrifuges, digital scales with different accuracies, pH meter, bomb calorimeter, etc.
Soxhlet extractor: This device is used to separate fats (lipids) from solid materials. The extracted fat materials can be sent to the central laboratory for additional GC or GC-Mass testing to determine the fatty acid profile and its active ingredient. The type of device available in the precipitation system group is made by the German Behr brand.
Equivalent to 1 gram of dry ground samples were poured into bags (6 x 4 cm with holes of 25 micrometers in diameter) and the lids were sewn with a thermal sewing machine. Approximately 3600 ml of neutral detergent insoluble fiber solution or acidic detergent insoluble fiber solution was poured into the tank of the device and the device was turned on. The device timer was set to 75 minutes (15 minutes to reach a temperature of 100 degrees Celsius and 60 minutes to boil the samples in the detergent solution). After draining the digestion solution, 4000 ml of hot distilled water is poured into the tank and the bags are washed in boiled distilled water for 5 minutes again. After that, the tank is emptied and the door of the machine is opened. After draining the water, the bags are dried in a container containing acetone and then between two layers of clean cloth. Then they are dried for 12 hours at a temperature of 105 degrees Celsius and weighed after cooling. The percentage of insoluble fibers in neutral or acidic detergent of the samples is calculated using the difference.
